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Bacillus Subtilis Bacteria Impregnation in Concrete for ...

Bacillus Subtilis Bacteria Impregnation in Concrete for Enhancement in Compressive Strength Koustubh A. Joshi 1, Madhav B. Kumthekar 2, Vishal P Ghodake3 1P.G. Student, Department of Civil Engineering, TKIET, Warananagar, Maharashtra, India 2 Professor, Department of Civil Engineering, Government Engineering College, Karad, Maharashtra, India

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(PDF) Application of Bacillus Subtilis Bacteria for ...

The cracks repair in the concrete cube using B. sphaericus was investigated [34]. In the previous study using B. subtilis in concrete and cement mortar were investigated compressive strength ...

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(PDF) Review on Self-Healing Concrete with Bacillus subtilis

Bacillus subtilis is an ideal bacterial spe cies. for self-healing concrete. Bacterial concrete claims to have a highe r tensile strength, compressive strength, high water permeability, and dur ...

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Bacillus subtilis bacteria used in fiber reinforced ...

Jan 10, 2020  In order to prevent sulfate ion penetration into concrete, Nosouhian et al. (2015) subjected their specimens to surface treatment with the calcium carbonate sediments formed by the three bacteria Sporosarcina pasteurii, Bacillus subtilis, and Bacillus sp. to find that the bacterially treated specimens showed not only better resistance against ...

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Bacillus subtilis bacteria used in fiber reinforced ...

In the present study, use was made of bacteria as sediment producing agents in concrete specimens strengthened with different types of fiber. For this purpose, a strain of Bacillus subtilis was employed in its culture medium at a concentration of 10 7 cell/ml both as a replacement for the water in the concrete mixture and in a surface treatment ...

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Study of Self Healing Mechanism and its Impact on Bacillus ...

this study bacteria called bacillus subtilis was impregnated in concrete and study was focused on its self healing mechanism and its impact on concrete properties such as compressive strength, surface velocity. 2. BACILLUS SUBTILIS Bacillus subtilis was originally named as Vibrio subtilis and renamed in 1872, this organism has other names

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Bacillus Subtilis - Bactaheal-PR Self Healing Concrete ...

White. Melting Point. 150 Degree. BACTAHEAL- PR. (Proven Enzyme, Bacterial formulation for Self healing concrete) Introduction: Prions Biotech offers enzyme blend BACTAHEAL-PR. The BACTAHEAL-PR is a new concept in the cement industry. Cracks in concrete are a common phenomenon due to

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Bacterial Concrete or Self Healing Concrete For Crack

Bacterial concrete or self healing concrete fills up the cracks developed in structures by the help of bacterial reaction in the concrete after hardening. Types of bacteria, its mechanism and preparation of bacterial concrete is discussed. In modern days, the use of technology has taken the standards of construction to a

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The Utilization of Bacillus Subtilis Bacteria to Improve ...

Nutrient Broth (NB) media was employed for the growth and spore formation of Bacillus subtilis bacteria in this experimental study and four different bacterial culture densities including 0.107, 0.2, 0.637, and 1.221 were estimated at OD600 and directly added to the concrete matrix

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Mechanical and Durability Characteristics of Bacterial ...

Dec 17, 2019  Bacterial concrete is a solution for the micro cracks. Bacterial concrete has a self healing property by which it can repair cracks upto 0.5 mm width. An investigation is conducted to study changes in the compressive strength, split tensile strength and flexural strength of concrete by the addition of bacteria.

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AN EXPERIMENTAL STUDY ON SELF HEALING OF CONCRETE

Meera Subha (2016) studied on the strength and durability assessment of bacteria based self-healing concrete using Bacillus subtilis bacteria. It was found that the bacteria based self-healing process healed cracks completely up to 0.5 mm width. On the surface of control concrete, calcium carbonate forms due to

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Performance of Microbial Concrete Developed Using Bacillus ...

Bacillus subtilis JC3, a common soil bacterium, has inherent ability to precipitate calcite crystals continuously which enhances the strength and durability performance of concrete enormously. This microbial concrete can be called as a "Self healing Bacterial Concrete" because it can remediate its cracks by itself without any human intervention ...

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Bacillus Subtilis - Bactaheal-PR Self Healing Concrete ...

White. Melting Point. 150 Degree. BACTAHEAL- PR. (Proven Enzyme, Bacterial formulation for Self healing concrete) Introduction: Prions Biotech offers enzyme blend BACTAHEAL-PR. The BACTAHEAL-PR is a new concept in the cement industry. Cracks in concrete are a common phenomenon due to

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Evaluation of Strength and Durability Properties for ...

Jun 19, 2020  Bacillus subtilis. Bacillus subtilis is a bacteria used in bio-concrete. This is a spore-forming, motile, rod-shaped, Gram-positive, facultative aerobe. It is predominantly found in soil and vegetation with an optimal growth temperature from 25-35 degrees Celsius.

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Experimental Study on Bacterial Concrete

To verify the performance of bacillus subtilis with 1mm and 2mm crack width and 15mm, 20mm, 25mm, and 30mm crack depth. III. LITERATURE REVIEW V. Ramakrishnan [1] The concept of bacterial concrete was first introduced by V. Ramakrishnan, (USA) a novel technique in remediating cracks in

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Volume 7 Issue 6, December 2017 Bacterial Concrete: A Review

organism was renamed Bacillus subtilis in 1872. POSITIV Bacillus Subtilis bacteria were one of the first bacteria to be studied. There cells are rod-shaped, Gram-positive bacteria that are naturally found in soil and vegetation. Bacillus subtilis grow in the mesophilic temperature range.

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Experimental study on characteristics of bacterial concrete

2.5 Bacillus Subtilis Bacteria Bacillus subtilis will be brought up in its log phase in concreting site in liquid or aqueous state. This stage is having bacterial concentration 2 x 108 cells/ml. This full-grown stage is aimed to lasts for 2 to 3 hours at room temperature. These bacteria should be impregnated in

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Bacteria as Self-Healing Agent in Mortar Cracks

impregnation of bacterial spores into pulverized fly ash medium to protect the bacteria in mortar mixture with high alkaline conditionthe s. Bacillus subtilis. has been studied for use in bacterial concrete to improve the strength of mortar [7], but its use self-healing concretein has not yet been studied extensively.

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Bacterial Concrete or Self Healing Concrete For Crack Repairs

Bacterial concrete or self healing concrete fills up the cracks developed in structures by the help of bacterial reaction in the concrete after hardening. Types of bacteria, its mechanism and preparation of bacterial concrete is discussed. In modern days, the use of technology has taken the standards of construction to a

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(PDF) STUDY ON DURABILITY PROPERTIES OF BACTERIAL CONCRETE

Influence of bacteria on the properties of concrete made with rice husk ash (RHA) is presented in this paper. For this purpose, control concrete was designed to have 28-d strength of 32.8 MPa. In ...

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EFFECT OF BACILLUS SUBTILIS ON MECHANICAL BEHAVIOR

micro-organism (Bacillus subtilis) incorporated into the concrete and calcium lactate and nutrient broth food to support those micro-organisms once they become active [2]. The micro-organism, feeding on the provided food supply, heal the crack. This paper also will make a case for, in-depth, the method that extent part behind micro-

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BACILLUS SUBTILIS - AND THERMUS THERMOPHILUS - DERIVED ...

Bacteria can precipitate mineral through biomineralisation which can be exploited to increase the strength of concrete. In this study, two types of bacteria, namely Bacillus subtilis and Thermus thermophilus, were used to produce bioconcrete. These bacteria are able to precipitate silica minerals when incubated in silicic acid solution. The silica minerals subsequently react with calcium ...

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Performance Of Bacterial Concrete Embedded With Bacillus ...

Too much professionalism Performance Of Bacterial Concrete Embedded With Bacillus Subtilis Sunil Pratap Reddy Syamla and exactly how i wanted. I think 5 stars are less for Performance Of Bacterial Concrete Embedded With Bacillus Subtilis Sunil Pratap Reddy Syamla

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Vol. 5, Issue 8, August 2016 Durability and Self- Healing ...

In bacterial concrete, due to urease enzymatic activity of Bacteria bacillus subtilis produces urease, which catalyzes urea to produce CO 2 2and ammonia, resulting in an increase of pH in the surroundings, Ca 2+ and CO 3-ions precipitates as calcite. Calcite production also takes place due to metabolism of bacteria. For that process it consumes ...

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BACTERIAL CONCRETE : Development OF Concrete To Increase ...

Results have been compared with conventional concrete. Biological modifications of construction materials are the need of the hour for strength improvement and long term durability. The present study propose to Increase compressive, tensile strength and self healing for concrete. Keywords: Bacteria, Bacillus Subtilis, Self- healing, durability.

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Experimental data of bio self-healing concrete incubated ...

The mortar was impregnated with Bacillus subtilis and this bacteria was encapsulated in calcium alginate for protection from the production process. The mortar specimens were mechanically cracked and then incubated within fine-grained fully saturated natural soil for about 4 weeks.

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Volume 7 Issue 6, December 2017 Bacterial Concrete: A Review

organism was renamed Bacillus subtilis in 1872. POSITIV Bacillus Subtilis bacteria were one of the first bacteria to be studied. There cells are rod-shaped, Gram-positive bacteria that are naturally found in soil and vegetation. Bacillus subtilis grow in the mesophilic temperature range.

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Experimental Investigation on Properties of Bacterial Concrete

specimen was made with addition of Bacillus Subtilis bacteria alone; whereas in the other set of specimen a Consortium of bacteria was used which consist of all the bacteria incuding Bacillus Subtilis. Fig. 1 Bacterial solution prepared to be added in concrete

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A Study of Microorganism (Bacteria) on Concrete Strength ...

days by addition of bacillus subtilis bacteria when compared to conventional concrete. The addition of bacillus subtilis bacteria showed significant improvement in the split tensile strength than the conventional concrete. From the durability studies, the percentage weight loss and percentage strength loss with 5%

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A Critical Review on Bacterial Concrete

Bacterial concrete is a material, which can successfully remediate cracks in concrete. ... impregnation of cracks with epoxy based fillers, latex ... Types of bacteria used in concrete are Bacillus subtilis Bacillus pseudofirmus, Bacillus pasteurii, Bacillus sphaericus, Escherichia coli, Bacillus cohnii, Bacillus ...

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Compressive Strength Improvement and Water Permeability of ...

Keywords: Self-Healing Concrete, Calcium Carbonate, Crack, Bacteria, Bacillus Subtilis Natto. 1 Introduction Although concrete is probably the most commonly used construction material, cracking in concrete structure has been experienced by both the research community and the industrial engineers.

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PAPER OPEN ACCESS Improvement Properties of Self -Healing ...

May 19, 2020  Bacteria Bacillus Subtilis under the micrographic. 3.2 Concrete mixes Four concrete mixes were intended in this research included, control concrete (reference mix) and three concrete mixes including, the different concentration of solution bacteria (103, 106 and 109) cell / mm. 3.3 Mix proportion

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The Utilization of Bacillus Subtilis Bacteria to Improve ...

Nutrient Broth (NB) media was employed for the growth and spore formation of Bacillus subtilis bacteria in this experimental study and four different bacterial culture densities including 0.107, 0.2, 0.637, and 1.221 were estimated at OD600 and directly added to the concrete matrix

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Experimental Investigation on Bacterial Concrete Using ...

special concrete known as bacterial concrete or otherwise called as self-healing concrete where the bacteria is induced in the mortars and concrete to heal up the faults. Researchers with different bacteria proposed different concrete. Here an attempt was made by using the bacteria “Bacillus Subtitles”.

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What is Bacterial Self Healing Concrete? Its History ...

Jul 21, 2020  Bacterial self-healing concrete is an innovative technology allowing repairing open micro-cracks in concrete by calcium carbonate (CaCO3) precipitation. This biotechnology improves the durability of the structure. Abstract Nowadays alternative approaches in construction technology are evolving rapidly in a bid to crucial the environmental hazards and low sustainability imparted by the ...

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Microorganism, Carriers, and Immobilization Methods of the ...

For Bacillus subtilis, it has a higher yield of biofilm on the bacterial surface and better repair efficiency with a median crack healing diameter of 1.8 mm. In addition, Bacillus subtilis results in a 22% increase in compressive strength and a 7% increase in flexural strength . In addition, Bacillus subtilis has a good performance in the ...

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Article: Effect of calcium lactate and Bacillus subtilis ...

Scanning electron microscope analysis shows that the dense matrix is obtained in bacterial concrete as compared to the control mix. The significant findings of this examination affirm that the performance of concrete improved by adding calcium lactate and Bacillus subtilis bacteria into the concrete.

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ELEMENTS - A self-healing construction material

The selected strain of Bacillus subtilis is an especially tough representative of the hay bacteria group. These bacteria exist almost everywhere, and they thrive just as well in the soil as in the human gut. However, concrete is an inhospitable environment for these microorganisms.

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